Alkaline Phosphatase, Calf Intestinal (HC) from Promega

Product Specs
ItemAlkaline Phosphatase, Calf Intestinal (HC)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM2825
SpeciesCalf
Conjugate/Tag/LabelUnconjugated
Quantity1,000u
Molecule NameAlkaline Phosphatase, Calf Intestinal (HC)
Concentration20u/µl

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1µmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidineMolecular cellAugust 4, 2022Arango D, Sturgill D, Yang R, Kanai T, Bauer P, Roy J, Wang Z, Hosogane M, Schiffers S, Oberdoerffer S.and dephosphorylated using 0.5 U/μl of Calf Intestinal Alkaline Phosphatase (CIAP, Promega, Cat. #: M2825) and 5’ phosphorylated with T4 Polynucleotide Kinase (PNK, New England Biolabs, NEB, Cat. #: M0201). For

(2) Human neuroblastoma SH-SY5Y cells treated with okadaic acid express phosphorylated high molecular weight tau-immunoreactive protein speciesJournal of Neuroscience MethodsMay 1, 2019Mirta Boban, Mirjana Babić Babić Leko, Terezija Miškić, Patrick R. Hof, Goran Šimićsupernatant (10 μl) was incubated with 10 U of alkaline phosphatase (Promega, Fitchburg, WI, USA, cat. no. M2825) or water (control) at 37°C for 30 min, followed by addition of Laemmli buffer and 5 min incubation

(3) BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaksNature CommunicationsAugust 1, 2017Winston X. Yan et al.in the presence of 0.015 U μl–1 of calf intestinal alkaline phosphatase (Promega, catalogue number M2825) before in situ ligation. Image processing and counting of γH2AX foci and cells All algorithms were

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